Coronaviruses 101: Focus on Molecular Virology

INTRO 0:19 - Human CoVs; CoV spillover to humans 5:13 - SARS vs. COVID19 8:45 - Talk overview 1. ENTRY 10:10 - CoV structure; spike conservation 14:41 - Spike and ACE2 interaction; protease cleavage 18:30 - CoV-2 spike residues 2. REPLICATION & GENE EXPRESSION 20:08 - 2019-nCOV annotation; 14 ORFs and 27 proteins 24:05 - Ribosomal frameshifting 26:38 - Sub-genomic mRNAs with shared 5' and 3' 29:06 - Shared transcription regulatory sequences 32:13 - CoV replicase; polymerase, capping and proofreading 34:08 - Exonuclease; viruses with >20kb genome 36:14 - Loss of Exonuclease; lethal mutagenesis 38:58 - Exonuclease and methyltransferase 3. Replication-transcription complexes (RTCs) 40:54 - Interconnected double membrane vesicles 43:32 - Vesicle formation 44:32 - Proximity labelling; Characterizing RTC proximal proteome 50:20 - Accessory genes 52:01 - Assembly of nucleocapsids into virions 4. Immune interactions 53:31 - Little or no interferon in cells; delayed interferon singling 57:39 - Short lived antibody and memory B cell response OUTRO 59:38 - Key open basic science questions

I took a virology course in graduate school almost a decade ago, what an incredible distance we have come. As a microbiologist myself I really appreciate putting your lecture up.

Thank you for this. I am a Candian biochemist stuck in Brazil. I decided to help the local authorities out. This brings me up to speed. I shared it among the English-speaking doctors as well.

Despite having no background in medicine or biology etc, I found this lecture really informative and easily understandable

The manner in which material is presented is exceptional. To pack such amount of information in just one lecture is evidence of years of teaching the best students.This is an excellent example of excellent teaching for all teachers in academia

Trust me. This whole hour of the video is worth watching. The professor details every key note about coronavirus. (I am not a virologist).

It took me complete 4 hours 45 min to understand this 1 hour lecture

As an undergrad biology major, I really enjoyed this in depth and straight forward overview. Thank you for making and uploading this and allowing me to apply and enhance my knowledge!

Thank you Dr. Glaunsinger.

Thank you, Dr. Glaunsinger, for that excellent introduction.

What a beautiful, clear minded, articulate, logical, level, educated, balanced, reasoned and highly intelligent voice. Very many thanks!! A perfect lecture.

Prof. G. Thank you for the wonderful, insightful presentation. I have to point out one thing. ACE 2 is an enzyme and has substrates (angiotensin 1 and 2). There is no ACE 2 receptor. SARS Covid 2 uses ACE 2 as a "receptor" to enter the cell. So ACE2 is a "receptor" for SARS Covid 2 and one may call ACE2 an SARS Covid 2 receptor. It actually functions as an acceptor. Pharmacologically, a receptor, upon being bound by a ligand, should trigger a cascade of biochemical/physiological reactions. Virologists use the term receptor differently.

Many thanks for this. As a clinician, this sort of exposition is incredibly helpful in gaining a better understanding of what we are trying to manage (treat?). The link between basic science and clinical medicine is always a challenge and this sort of background knowledge is invaluable when thinking about what we are seeing in patients.

This explanation was amazing! I'm an undergraduate student, only in my 2nd semester of biochem and was able to keep with a lot of it because it was very well explained. I loved it, thank you, and also thank you to all the scientists working tirelessly to fight this.

Well done. Refreshing to see some virology 101 long-form presentations to counter a lot of the misinformation out there.

Great lecture. Very informative. I would love for Dr. Glaunsinger to give a follow-up lecture. Now that it has been almost two months with 10x more cases and more information I would love to hear her take on what's happening now.

Thank you, Dr. Glaunsinger, for this lecture

Thank you, Britt, for producing this outstanding review. I'm sending the link out to our colleagues. (Dave Sulzer, Columbia Medical School)

An Outstanding Lecturer. Thank You Professor Britt Glaunsinger.

Studying viral biology at UG level. This is a really straight and detailed presentation. Thank you.